ABSTRACT
Objective To investigate the expression of T-cell subset,changes of TNF-?,IL-6,IL-8,sIL-2R levels in the sera and its role in development of chromic viral hepatitis B,in order to understand viral replication and clinical significance in patients with chronic hepatitis B(CVHB).Methods Alkaline phosphatase/antialkaline Phosphatase(APAAP) and ELISA techniques were used to detect the levels of T-cell subset in peripheral blood and serum TNF-?,IL-6,IL-8,sIL-2R in 290 patients with chronic hepatitis B.Results In the count of CD 4 + cell and CD 4 +/CD 8 + were significanyly lower,and in the count of CD 8 + cell and levels of TNF-?,IL-6,IL-8,sIL-2R in CVHB patients group were higher than in normal control group(P
ABSTRACT
Objective To explore the relationship between serum cytokines and liver fibrosis in patients with chronic hepatitis B(CHB), and to discuss their role in the pathogenesis of liver fibrosis. Methods ELISA and RIA techniques were used to detect the levels of transforming growth factor-?1( TGF-?1),tumor necrosis factor-?(TNF-?), Interferon -?(IFN-?), hyaline acid (HA), type III procollagen (PC Ⅲ) and collagen type Ⅳ(C-Ⅳ) in 95 patients with CHB, and contrast study were performed between those serum markers and hepatic pathology. Results The levels of serum TGF-?1, TNF-?, HA, PCⅢ and C-Ⅳ in the patients with CHB were significantly higher than those in normal control group (NC), and were correlated with degree of liver damage positively. Serum levels of TGF-?1, TNF-?, HA, PCⅢ and C-Ⅳ were positive proportion to degree of liver fibrosis (P